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Hinkle Lab

Welcome to the Hinkle Lab

Professor Emeritus, Pharmacology and Physiology

Research Overview

We are interested in the broad question of how a hormone triggers a characteristic set of cellular responses. We use a number of model systems (pituitary cells, cell lines, transgenic animals) to study signal pathways for a hypothalamic peptide hormone, TRH, which acts via a G protein coupled receptor to increase cytoplasmic calcium and protein kinase C activity. Modern technologies allow us to study hormonal responses in live cells. For example, we can "tag" signaling proteins with green fluorescent protein (GFP) and then follow their movements in the cell during hormonal stimulation in real time. We monitor intracellular calcium in the cytoplasm and the endoplasmic reticulum using several fluorescence imaging techniques. In addition, we use biochemical and genetic approaches to identify proteins that interact with the receptor during biosynthesis, signaling and desensitization. Similar strategies are being used to ask how insulin secretion by the pancreatic beta cell is controlled. The long-term goal is to understand signal transduction in endocrine cells at a molecular and cellular level.

Movie showing the movement of GFP-beta-arrestin after TRH additionThe movie shows the movement of GFP-beta-arrestin after TRH addition. When TRH binds to its receptor, the receptor changes conformation, activates Gq, and becomes phosphorylated by specific receptor kinases; the phospho-receptor binds to beta-arrestin. The movie covers about 10 min.

Patricia M. Hinkle, Ph.D.

Patricia M. Hinkle, Ph.D.
Principal Investigator

Publications

    1. Gehret AU
    2. Hinkle PM
    ; ACS chemical biology; Vol 8(3). 2013 Mar 15.
    1. Thal DM
    2. Homan KT
    3. Chen J
    4. Wu EK
    5. Hinkle PM
    6. Huang ZM
    7. Chuprun JK
    8. Song J
    9. Gao E
    10. Cheung JY
    11. Sklar LA
    12. Koch WJ
    13. Tesmer JJ
    ; ACS chemical biology; Vol 7(11). 2012 Nov 16.
    1. Christian WV
    2. Li N
    3. Hinkle PM
    4. Ballatori N
    ; The Journal of biological chemistry; Vol 287(25). 2012 Jun 15.
    1. Hinkle PM
    2. Gehret AU
    3. Jones BW
    ; Frontiers in neuroscience; Vol 6. 2012.

View All Publications

Contact Us

  Hinkle Lab
Box 711
601 Elmwood Ave
Rochester, NY 14642

 (585) 275-4933

  (585) 273-2652